RNA

Part:BBa_K2412000:Design

Designed by: Jennifer Fang   Group: iGEM17_ColumbiaNYC   (2017-10-14)


eGFP shRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is difficult to assemble because the shRNA has a hairpin structure, so we used USER cloning to assemble the part.


Source

The sequence of this part is derived from the sequence of commercialized eGFP.

References

The shRNA sequence was published on https://www.addgene.org/30323/