RNA
Part:BBa_K2412000:Design
Designed by: Jennifer Fang Group: iGEM17_ColumbiaNYC (2017-10-14)
eGFP shRNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is difficult to assemble because the shRNA has a hairpin structure, so we used USER cloning to assemble the part.
Source
The sequence of this part is derived from the sequence of commercialized eGFP.
References
The shRNA sequence was published on https://www.addgene.org/30323/